Protein_Domain

Part:BBa_K3582102:Design

Designed by: Avadhoot Jadhav, Merrin Vincent   Group: iGEM20_IISER-Pune-India   (2020-10-20)


CIDRa domain from IT4var07 PfEMP1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • 1] RFC10 criteria satisfied
  • 2]6xHis tag gene added for purification.
  • 3]Codon optimized with the E-coli K-12 strain.


Source

Reverse Translation

References